GS Discovery® – Benchmarking Report

As part of the Research Evaluation Agreement, we request that the data below is supplied in order to provide information as required in the agreement. The purpose of this report is to understand your experience of the GS Discovery® transient gene expression system and whether the process met your expression needs.

Thank you for your cooperation!

This report assumes that you have used the GS Discovery® transient gene expression system as described in the GS® manual portal and in combination with the GS Xceed® CHOK1SV GS-KO® host cell line.

Name Position Company Department Email

Which GS Discovery® transient process did you use? with GS piggyBac® and pTGE23 without GS piggyBac® and pTGE23 modified process

Note: if multiple processes were tested, please provide data on all.

If you have made any modifications to the process, for example to the transfection process itself, the vectors, the media, or any other parameters, please can you provide details below. If you made any changes to the process, can you comment on why such changes were made? Did the GS Discovery® transient gene expression system allow you to reach your transient gene expression goals?

In general, how do you rate your experience of the GS Discovery® transient gene expression system? (1 = excellent, 5 = poor) 1 2 3 4 5

Please can you provide some additional details on why you selected the score above? Did you compare the performance of the GS Discovery® transient gene expression system with any other commercially-available transient gene expression systems? If yes, which ones did you test, and can you comment on the relative performance of the Lonza platform? Please provide details of the molecule(s) being expressed. Please tick any of the terms below which describe the molecule being expressed, or if not listed, please provide a description of the molecule type(s): Molecule name or identifier: IgG1 IgG2 IgG4 Aglycosyl bsAb IgG bsAb IgG engineered to solve heavy chain pairing issue bsAb IgG engineered to solve light chain pairing issue Fragment based upon scFv fragments eg BiTes Fragment - based upon single domain antibodies Fusion protein Non-mAb glycoprotein Other If using a bispecific pairing solution, please indicate which one below:

Where data is available, please complete all of the below sections.

Note: Where data is available from alternative expression systems, please provide comparative data.

Vector Construction

Did you use the GSquad® vector system for expressing your product genes?

If not, please detail which vectors were used below: If your product consisted of more than one product gene, did you test any different chain order configurations (5’ – 3’) in the vector backbone?

If yes, please provide details:

Transient transfection

Vessel type (scale e.g. plate or flask) Viability post-transfection Titer at harvest Length of culture (time) Maximum viable cell concentration Harvest viable cell concentration Viability at harvest (%)

Product quality (PQ)

Were you happy with the quality of the material generated using the GS Discovery® system?

Were the post-translation modifications performed correctly on your product?

If no, please can you provide additional details?

Product quality attributes – aggregation:

Were high molecular weight forms observed at more than 5%?

Product quality attributes – glycosylation:

Were high mannose structures observed at more than 5%? Percentage G0F and G1F observed? Was the sialylation profile satisfactory?

Product quality attributes – correct assembly of the product:

Proportion of the product that was correctly assembled (%)? Would you like us to contact you to have a technical discussion?

By submitting your data through this form, you confirm that you are above the age of 18, that you have read and understood the Privacy Policy, and that you agree to the collection, use and processing of your Personal Information by Lonza in accordance with said policy.